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Identification of the transcriptional regulatory gene of histidase and urocanase in Pseudomonas aeruginosa -Regulation of histidase and urocanase expression-
https://aino.repo.nii.ac.jp/records/356
https://aino.repo.nii.ac.jp/records/3568bebdebb-d12d-47eb-9d98-b7ccc58def15
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00007506779.pdf (356.7 kB)
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| Item type | [ELS]紀要論文 / Departmental Bulletin Paper(1) | |||||||
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| 公開日 | 2019-03-27 | |||||||
| タイトル | ||||||||
| タイトル | Identification of the transcriptional regulatory gene of histidase and urocanase in Pseudomonas aeruginosa -Regulation of histidase and urocanase expression- | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Pseudomonas aeruginosa | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | repressor | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | histidase | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | urocanase | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| 雑誌書誌ID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA11877461 | |||||||
| ページ属性 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | P | |||||||
| 論文名よみ | ||||||||
| タイトル | Identification of the transcriptional regulatory gene of histidase and urocanase in Pseudomonas aeruginosa -Regulation of histidase and urocanase expression- | |||||||
| 著者 |
NAKADA, Yuji
× NAKADA, Yuji
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| 著者所属(英) | ||||||||
| en | ||||||||
| Department of Nursing, Faculty of Nursing and Rehabilitation, Aino University | ||||||||
| 記事種別(英) | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Original | |||||||
| 抄録(英) | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Pseudomonas aeruginosa is a typical opportunistic bacterial pathogen that can grow under poor nutrient conditions. Histidine utilization as a sole source of carbon and nitrogen is a distinctive feature of P.aeruginosa, which decompose and utilize a variety of nutrients and complex compounds using various catabolite pathways. When wild-type P.aeruginosa PAO1 cells were cultured in minimal medium P (MMP) containing 20mM histidine, synthesis of histidase and urocanase was detected in crude extract at 3186±118 (U/mg-proteins) and 243±3 (10-3 U/mg-proteins), respectively. It was determined that the synthesis of these enzymes was induced by histidine because limited enzyme synthesis was observed in MMP containing 20mM of glutamate. In contrast, PAO4399, the spontaneous mutant of PAO1, can synthesize histidase and urocanase sufficiently under the nutrient conditions of MMP containing histidine or glutamate. A nucleotide sequence analysis of PAO4399 showed a C to G transition at nt 441 of the PA5105 gene, with this mutation causing an amino acid change of the tyrosine codon (TAC) Tyr 147 to stop codon (TAG). In the case of PAO4816, the knockout mutant strain of PAO1 with an inserted gentamicin (Gm)-cassette in the PA5105 gene, the cells grown in MMP containing 20mM of histidine or glutamate synthesized histidase and urocanase constitutively, in the same manner as PAO4399. The PA5105 gene product is highly homologous with the hutC gene product of Pseudomonas putida, which regulates histidase and urocanase gene expression. These findings support the role of the PA5105 gene of P.aeruginosa PAO1 as a repressor-type hutC gene that regulates histidase and urocanase synthesis under histidine-dependent nutrient conditions. | |||||||
| 書誌情報 |
en : Aino journal 巻 8, 発行日 2010-03-31 |
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| 表示順 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 6 | |||||||
| アクセション番号 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | KJ00007506779 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 1348480X | |||||||
